John S. Lumsden

BSc, DVM, MSc, PhD, MACVSc, Diplomate ACVP

jsl@uoguelph.ca

Research Interests

Aquatic Animal Health

Innate immunity, regeneration and proteomics - teleosts and corals

Hosts of interest; O. mykiss, S. salar, M. cavernosa,R. florida

Agents of particular interest; Flavobacterium psychrophilum, Tenacibaculum sp. and viral hemorrhagic septicemia virus (VHSV) IVb

Current Graduate Students

Doctoral

• Zach Millar - PhD
• Joseph Nowlan - PhD

Master's

• Scott Britney - MSc
• Stephanie Gunby - MSc
• Dimo Kecheliev - MSc
• Brianna Heese - MSc

Former graduate students

• Joseph Nowlan, MSc, 2020. Quantitative PCR detection of Tenacibaculum maritimum and T. dicentrachi at netpen sites in British Columbia Canada.
• Juan-Ting Liu, PhD, 2019. Autophagy and resistance to VHSV IVb in RTgill-W1 and Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss).
• Ryan Eagleson, MSc, 2019. Spatial distribution of benthic habitats and ecological patterns of the mustard hill coral (Porites astreoides) in the nearshore waters of Grenada.  
• Maureen Jarau, PhD, 2018. Virulence of Flavobacterium psychrophilum, antimicrobial treatment and intestinal microbiota of rainbow trout (Oncorhynchus mykiss).
• Ryan Horricks, PhD, 2017. Tissue regeneration of artificially induced lesions in the Caribbean great star coral (Montastraea cavernosa) in the nearshore waters of Grenada.
• Jaramar Balmori-Cedeno, MSc, 2017. Autophagy-related (Atg) genes in rainbow trout (Oncorhynchus mykiss) and their role in nutrient restriction in vivo and in vitro.
• Paige Vroom, MSc, 2016. Regeneration in Corallimorpharia.
• Doran Kirkbright, DVSc, 2016. Quantitative PCR for epitheliocystis in lake trout (Salvelinus namaycush).
• Ehab Misk, PhD, 2016. Pathogenesis and distribution of spring viaemia of carp virus in Ontario.
• Adrian Di Natale, MSc, 2016. Apolipoprotein domain containing proteins and innate immunity in rainbow trout and walleye.
• Ian Ryerse, MSc, 2014. Deoxynivalinol in rainbow trout (Oncorhynchus mykiss).
• Elena Contador, MSc, 2014. Epitheliocystis in Lake trout (Salvelinus namaycush).

Research Associates, Post-docs, staff.

• John Pham. Research Technician. 2019-

Teaching

PATH*4100 Diseases of Aquatic Animals

VETM*3120 Histology and General Pathology

VETM*4480 Comparative Medicine

Project students in IBIO 4500, 4521 and BIOM 4510. 2022-2023.

Julie-Ann Arbour - Microplastics in Rhodactis sp.

Taylor Groothuis - Impact of flatworm infection on Rhodactis sp.

Tabitha Mirza - Impact of flatworm infection on Rhodactis sp.

Sandra Jaskowiak - Microplastics in Ricordea florida andRhodactis sp.

Brieanna Wagler - Microfragmentation and regeneration of scleratinian corals. 

Daniel Carr - Seahorse cell line SH3fin and viral competency.

Selected Publications

• Millar Z, Kecheliev D, Wolvin S, LePage V, Lumsden JS. 2023. Residual tissue magnesium concentration in jellyfish (Aurelia aurita and Cassiopea andromeda) following magnesium chloride euthanasia. Zoo Biology, Accepted.
• Eagleson R, Alvarez-Filip L, Lumsden JS. 2023. Changing Reefs of the Caribbean: A biological review of the scleractinian coral Porites astreoides. Diversity 2172700.
• Horricks RA, Millar Z, Sykes K, Lumsden JS. 2023. Differential protein abundance in the regenerating Caribbean corallimorpharian, Ricordea florida. Journal of Zoo and Aquarium Research, 11:240-248. doi.org/10.19227/jzar.v11i1.700
• Nowlan JP, Sies AN, Britney SR, Siah A, Lumsden JS, Russell S. 2023. Genomics of Tenacibaculum species in British Columbia, Canada. Pathogens 12, 101. doi.org/10.3390/pathogens12010101.
• Huyben D, Jarau M, MacInnes JI, Stevenson R, Lumsden JS. 2023. Impact of infection with Flavobacterium psychrophilum and antimicrobial treatment on the intestinal microbiota of rainbow trout. Pathogens 1998414. doi.org/10.3390/pathogens12030454
• Nowlan JP, Heese BM, Wilson MJ, Britney SR, Lumsden JS, Russell S. 2022. Tenacibaculum ovolyticum 16s rDNA quantitative-PCR assay development and field testing. Fishes, 7(6), 303. doi.org/10.3390/fishes7060303.
• Evans D, Millar Z, Harding D, Pham PH, LePage V, Lumsden JS. 2022. Lipoid liver disease in Hippocampus erectus Perry with Vibriofortis-induced dermatitis and enteritis. Journal of Fish Diseases, doi.org/10.1111/jfd.13618.
• Balmori-Cedeno J, Liu J, Pham PH, Misk E, Ryerse I, Renshaw S, Nowlan J, Lumsden JJ, Lumsden JS. 2022. Autophagy-related gene regulation in the liver and muscle of rainbow trout Oncorhynchus mykiss Walbaum by exposure to chloroquine, deoxynivalenol and nutrient restriction. Aquaculture Research, doi:10.1111/ARE.15896.
• Misk E, Huber P, MacInnes JI, Sherif SM, Abo-Ismail M,Lumsden JS. 2022. Rainbow trout gill epithelium (RTgill-W1) innate response to ultraviolet-inactivated VHSV and FliC and to rhabdovirus  infection. Fish and Shellfish Immunology Reports, 100043.
• Liu J, Pham J, Lumsden JS. 2022. Autophagy modulation in rainbow trout (Oncorhynchus mykiss) and resistance to experimental infection with Flavobacterium psychrophilum. Journal of Fish Diseases, doi.org/10.1111/jfd.13578.
• Nowlan J, Britney S, Lumsden JS, Russell S. 2021. Experimental induction of tenacibaculosis in Atlantic salmon (Salmo salar L.) using Tenacibaculum maritimum, T. dicentrarchi and T. finnmarkense. Pathogens, 10-01439.
• Huyben D, Chaisson M, Lumsden JS, Pham J, Chowdhury MAK. 2021. Feeding a microencapsulated blend of organic acids and essential oils influences proximal intestinal fold length and gut microbiome of rainbow trout (Oncorhynchus mykiss). Microorganisms. 2076-2607/9/10/2063.
• Eagleson R,Lumsden JS, Alvarez-Filip L, Herbinger CM, Horricks RA. 2021. Benthic monitoring and local dynamics influencing the mustard hill coral (Porites astreoides) in the nearshore waters of Grenada. Diversity, 13, 288. doi.org/10/3390/d13070288.
• Shahmohamadloo RS, Hankins G, Hubbs K, Lumsden JS, Konopelko P, Sarnacki M, Strong D, Vander Eyken A, Ortiz Almirall X, Watson-Leung T, Simmons DBD, Bhavsar SP, Sibley PK. 2021. Differences in toxicodynamics between intracellular and extracellular microcystins in two life stages of rainbow trout (Oncorhynchus mykiss). Environmental Science and Technology. doi.org/10.1021/acs.est.1c01501.
• Nowlan J, Britney S, Lumsden JS, Russell S. 2021. Application of quantitative PCR to monitor netpen sites in British Columbia (Canada) for Tenacibaculum species. Pathogens, 10, 414. doi.org/10.3390/pathogens10040414.
• Evans D, Millar Z, Wolvin S, Pham PH, LePage V, Lumsden JS. 2021. Magnesium concentration influences pulse rate and growth in the upside-down jellyfish, Cassiopea andromeda. Journal of Zoo Biology, doi:10.1002/zoo.21631.
• Nowlan J, Lumsden JS, Russell S. 2021. Quantitative PCR for Tenacibaculum dicentrarchi and T. finnmarkense. Journal of Fish Diseases doi.org/10.1111/jfd.13357.
• Nowlan J, Lumsden JS, Russell S. 2020. Advancements in characterizing Tenacibaculum infections in Canada. Pathogens. 9(12), 1029; doi:10.3390/pathogens9121029.
• Pham PH, Papazotos F, Jones G, Misk E, Polinski MP, Contador E, Russell S, Lumsden JS, Garver KA, Bols NC. 2020. Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1) amplification: identification of the non-supportive PRV invitrome. Pathogens, 956065, doi:10.3390/pathogens9100833.
• Liu J, Lumsden JS. 2020. Impact of feed restriction, chloroquine and deoxynivalenol on viral hemorrhagic septicemia virus IVb in fathead minnow (Pimpheles promelas). Journal of Fish Diseases, doi.org/10.1111/jfd.13300.
• Horricks RA, Herbinger CM, Vickaryous MK, Taylor P, Lumsden JS. 2020. Differential protein abundance in delayed regeneration of the scleratinian coral Montastraea cavernosa. Coral Reefs doi 10.1007/s00338-020-01952-4.
• Liu J, Pham PH, Wootton S, Bols NC, Lumsden JS. 2020. VHSV IVb infection and autophagy modulation in the rainbow trout gill epithelial cell line RTgill-W1. Journal of Fish Diseases 43:1237-1247.
• Liu J, Balmori-Cedeno J, Pham PH, Misk E, Lumsden JS. 2020. Modulation of autophagy by deoxynivalenol and chloroquine in a rainbow trout Oncorhynchus mykiss gill epithelial cell line, RTgill-W1. In Vitro Cellular and Developmental Biology Animal, doi.10.1007/s11626-020-00490-1.
• Semple, SL, Bols NC, Lumsden JS, Dixon B. 2020. Understanding the pathogenesis of Flavobacterium psychrophilum using the rainbow trout monocyte/macrophage-like cell line, RTS11, as an infection model. Microbial Pathogenesis 139, 103910.
• Brown T, Evans D, Millar Z, Pham P, LePage V, Lumsden JS. 2020. Fusarium solani haplotype 12-b and aortic and branchial arteritis in Hippocampus erectus Perry. Journal of Fish Diseases 43:301-304.
• Horricks RA, Tabin SK, Edwards JJ, Lumsden JS, Marancik DP. 2019. Organic ultraviolet filters in nearshore waters and in the invasive Pacific lionfish (Pterois volitans) in Grenada, West Indies. PLoS ONE 14(7): e0220280.
• Di Natale A, Russell S, Jarau M, Lillie BN, Horricks R, Dixon B, Lumsden JS. 2019. Identification and partial characterization of type IV ice-structuring protein LS-12 in rainbow trout Oncorhynchus mykiss. Journal of Fish Diseases 42:975-984.
• Semple SL, Rodgiguez-Ramos T, Carpio Y, Lumsden JS, Estrada MP, Dixon B. 2019. Pituitary adenylate cyclase-activating polypeptide (PACAP) acts as an antimicrobial peptide and immunostimulant during Flavobacterium psychrophilum infections in RTS11. Frontiers in Immunology, doi:103389/fimmu.2109.00926.
• Hooft JM, Ferreira C, Lumsden JS, Sulyok M, Krska R, Bureau DP. 2019. The effects of naturally occurring or purified deoxynivalenol (DON) on growth performance, nutrient utilization and histopathology of rainbow trout (Oncorhynchus mykiss). Aquaculture, 505:318-9-332.
• Pham PH, Sokeechand BSH, Hamilton ME, Misk E, Jones G, Lumsden JS, Bols NC. 2019. VER-155008 impeded replication of two RNA viruses in fish cell cultures while concurrently inducing Hsp70 proteins. Antiviral Research, 162:151-162.
• Balmori-Cedeno J, Liu J, Misk E, Lillie B, Lumsden JS. 2019. Autophagy-related (Atg) genes in rainbow trout Oncorhynchus mykiss Walbaum and their role in nutrient restriction in RTgill-W1. Journal of Fish Diseases, 42:1-13.
• Horricks RA, Herbinger CM, Lillie BN, Lumsden JS. 2019. Differential protein abundance during the first month of regeneration in the Caribbean star coral Montastraea cavernosa. Coral Reefs, 38:45-61.
• Lumsden JS. 2019. Musculoskeletal Disorders. In. S Smith (Ed.). Fish Medicine. CRC Press. pp 149-172.
• Jarau M, MacInnes JI, Lumsden JS. 2019. Erythromycin and florfenicol treatment of rainbow trout Oncorhynchus mykiss Walbaum experimentally infected with Flavobacterium psychrophilum. Journal of Fish Diseases, 42:325-334.
• Jarau M, Di Natale A, Huber P, MacInnes J, Lumsden JS. 2018. Virulence potential of Flavobacterium psychrophilum isolates from Ontario, Canada in rainbow trout Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases, 41:1505-1514.
• Fraser RS, Lumsden JS, Lillie BN. 2018. Identification of polymorphisms in the bovine collagenous lectins and their association with infectious diseases in cattle. Immunogenetics 70:533-546.
• Pham PH, Sokeechand BSH, Garver KA, Jones G, Lumsden JS, Bols NC. 2018. Fish viruses stored in RNALater can remain infectious and even be temporarily protected from inactivation by heat or tissue homogenates. Journal of Virological Methods 253:31-37.
• Semple S, Kellendonk CJ, Al-Hussinee L, Lumsden JS, Dixon B. 2018. Serum IgM, MH class IIb genotype and respiratory burst activity do not differ between rainbow trout families displaying resistance or susceptibility towards the coldwater pathogen, Flavobacterium psychrophilum. Aquaculture 483:131-140.
• Lumsden JS.2017. Viral hemorrhagic septicemia virus. In. PTK Woo, R. Cipriano (Eds.) Fish viruses and bacteria: pathobiology and protection. CABI, pp 26-37.
• Pham PH, Tong WLW, Misk E, Jones G, Lumsden JS, Bols NC. 2017. Atlantic salmon endothelial cells from the heart were more susceptible than fibroblasts from the bulbus arteriosus to four RNA viruses but protected from two viruses by dsRNA pretreatment. Fish and Shellfish Immunology 70:214-227.
• Pham PH, Vo NTK, Tan E, Jones G, Lumsden JS, Bols NC. 2017. Development of an Atlantic salmon heart endothelial cell line (ASHe) that responds to lysophosphatidic acid (LPA). In Vitro Cellular and Developmental Biology Animal 53:20-32.
• Coffee LL, Getchell RG, Groocock GH, Cornwell ER, Wooster GA, Lumsden JS, Bowser PR. 2017. The pathogenesis of experimental viral hemorrhagic septicemia virus IVb infection in adult lamprey (Petromyzon marinus). Journal of Great Lakes Research 43:119-126.
• Misk E, Tubbs L, Garver K, Isaac S, Wright E, Al-Hussinee L, Lumsden JS. 2017. Spring viremia of varp virus: A RT-qPCR assay and surveillance in Ontario from 2008 to 2012. Journal of Great Lakes Research 43:127-131.
• Al-Hussinee L, Tubbs L, Russell S, Pham J, Tafalla C, Bols N, Dixon B, Lumsden JS. 2016. Temporary protection of rainbow trout gill epithelial cells from infection with viral hemorrhagic septicemia virus IVb. Journal of Fish Diseases 39:1099-1112.
• Misk E, Tubbs L, Isaac S, Al-Hussinee L, Huber P, Garver K, Nagy E, Lumsden JS. 2016. Pathogenesis of spring viremia of carp virus in emerald shiner Notropis atherinoides Rafinesque, fathead minnow Pimphales promelas Rafinesque and white sucker Catostomus commersonii (Lacepede). Journal of Fish Diseases 39:729-739.
• Contador E, Frasca S, Lillie B, Lumsden JS. 2016. Epitheliocystis in lake trout Salvelinus namaycush (Walbaum) is associated with a ß-proteobacteria. Journal of Fish Diseases 39:353-366.
• Vo NTK, Bender AW, Lumsden JS, Lorenzen N, Dixon B, Bols NC. 2016. Differential viral hemorrhagic septicemia virus genotype IVb infection in fin fibroblast and epithelial cell lines from walleye, Sander vitreus Mitchill, at cold temperatures. Journal of Fish Diseases 39:175-188.
• Kirkbright D, Lillie B, Huber P, Lumsden JS. 2016. Dermocystidium in yellow perch Perca flavescens Mitchill. Journal of Fish Diseases 39:597-601.
• Ryerse I, Hooft JM, Bureau DP, Hayes MA, Lumsden JS. 2016. Diets containing corn naturally-contaminated with deoxynivalenol reduces the susceptibilty of rainbow trout to experimental Flavobacterium psychrophilum infection. Aquaculture Research 47:787-796.
• Grice J, Reid A, Peterson A, Blackburn K, Tubbs L, Lord S, Huber P, Dixon B, Bols NC, Lumsden JS. 2016. Walleye Sander vitreus Mitchill are relatively resistant to experimental infection with viral hemorrhagic septicemia virus IVb and extant strains vary in susceptibility. Journal of Fish Diseases 38:859-872.
• LaPatra S, Al-Hussinee L, Misk E, Lumsden JS. 2016. Rhabdoviruses. In. F. Kibenge, M. Godoy (Eds.) Aquaculture Virology. pp.  Elsevier, Amsterdam, Netherlands. pp. 266-298.
• Ryerse I, Hooft JM, Bureau DP, Hayes MA, Lumsden JS. 2015. Purified deoxynivalenol and feed restriction reduces mortality in rainbow trout, Onchorhynchus mykiss Walbaum, with experimental bacterial coldwater disease but biologically relevant concentrations of deoxynivalenol do not impair the growth of Flavobacterium psychrophilum. Journal of Fish Diseases 38:809-819.
• Russell S, Tubbs L, LePage V, Young K, Huber P, Lumsden JS. 2015. Amyloidosis in two captive tricolour sharkminnows (Balantiocheilus melanopterus). Journal of Fish Diseases 38:561-565.
• Wu AKK, Kropinski AM, Lumsden JS, Dixon B, MacInnes JI. 2015. Complete genome sequence of the fish pathogen Flavobacterium psychrophilum ATCC 49418. Standards in Genomic Sciences 10:3 doi:10.1186/1944-3277-10-3.
• Vo NTK, Chen C, Lee LEJ, Lumsden JS, Dixon B, Bols NC. 2015. Development and characterization of an endothelial cell line from the bulbous arteriosus of walleye, Sander vitreus. Comp. Biochem. Physiol. A. 180:57-67.
• LePage V, Young J, Dutton C, Crawshaw G, Pare JA, Kummrow M, McLelland D, Huber P, Young K, Russell S, Al-Hussinee L, Lumsden JS. 2015. Diseases of captive yellow seahorse Hippocampus kuda Bleeker, pot-bellied seahorse Hippocampus abdominalis Lesson and weedy seadragon Phyllopteryx taeniolatus Lacepede. Journal of Fish Diseases 38:439-450.
• Vo NTK, Bender AW, Lumsden JS, Dixon B, Bols NC. 2015. Development of a walleye spleen stromal cell line sensitive to viral hemorrhagic septicemia virus and to protection by synthetic dsRNA. Fish & Shellfish Immunology 45:83-93.
• Vo NTK, Bender AW, Lee LEJ, Lumsden JS, Lorenzen N, Dixon B, Bols NC. 2015. Development of a walleye cell line and use to study the effects of temperature on infection by viral hemorrhagic septicemia virus group IVb. Journal of Fish Diseases 38:121-136.
• Kerr J, Lumsden JS, Russell S, Jasinska EJ, Goss G. 2014. Effects of anionic polyacrylamide products on gill histopathology in juvenile rainbow trout (Onchorhynchus mykiss). Environmental Toxicology and Chemistry 33:469-476.
• Sever L, Vo NTK, Lumsden JS, Bols N, Dixon B. 2014. Induction of rainbow trout MH class I and accessory proteins by viral hemorrhagic septicemia virus. Molecular Immunology 59:154-162.

Search PubMed for additional publications by Dr. Lumsden.