College of Biological Science
Department of Human Health and Nutritional Sciences
Office: ANNU 329A
Lab: ANNU 307
Dr. Clark’s main research focus is on the identification and characterization of chondrocyte signal transduction mechanisms that are initiated by the introduction of mechanical and chemical cartilage stressors. These transduction mechanisms enable chondrocytes to detect and respond to these changes in cartilage. Dr. Clark’s research to date has focused on the effects of proteins such as, TRPV4, integrin α1β1, and cilia on chondrocyte signal transduction. The long-term research goals of Dr. Clark include advancing the understanding of the molecular mechanisms which occur during osteoarthritis and to identifying new approaches to improve osteoarthritis treatments.
The Clark lab includes a lab technician and numerous graduate and undergraduate students receiving research training and experiences through MSc and PhD graduate programs, undergraduate research project classes, summer assistantships and work study programs. Together we manage a number of sophisticated genetic mouse models of integrin α1β1 and cilial deletion, while conducting individual research projects. These projects involve the use of confocal microscopy, histology, immunohistochemistry, reverse transcription polymerase chain reaction and micro computed tomography technologies. Dr. Clark values her international/national collaborations with Dr. Pozzi (Vanderbilt), Dr. Boyd (Calgary) and Dr. Vogel (Calgary) in addition to her partnerships at Guelph with Dr. Bent, Dr. Koch and Dr. Srbely.
Recent publications relating to regenerative medicine (2012-present):
1. Shin SY, Pozzi A, Boyd SK and Clark AL: (2016) Integrin α1β1 protects against signs of post-traumatic osteoarthritis in the female murine knee partially via regulation of epidermal growth factor receptor signaling. Osteoarthritis and Cartilage 24:10:1795-1806.
2. Mickiewicz B, Shin SY, Pozzi A, Vogel HJ and Clark AL: (2016) Serum metabolite profiles are altered by erlotinib treatment and the integrin α1-null genotype, but not by post traumatic osteoarthritis. Journal of Proteome Research 15:3:815-825.
3. Jablonski CL, Ferguson S, Pozzi A and Clark AL: (2014) Integrin α1β1 participates in chondrocyte transduction of osmotic stress. Biochemical and Biophysical Research Communications 445:1:184-190.
4. Parekh R, Lorenzo M, Shin SY, Pozzi A and Clark AL: (2014) Integrin α1β1 differentially regulates cytokine-mediated responses in chondrocytes. Osteoarthritis and Cartilage 22:3:499-508.
5. Rich DR and Clark AL: (2012) Chondrocyte primary cilia shorten in response to osmotic challenge and are sites for endocytosis. Osteoarthritis and Cartilage 20:8:923-930